产品名称

Glycopeptidase A from almonds

一般描述

Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine ages.

别 称

N-ed-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase, N-Glycosidase A, PNGase A

含 量

≥0.05unit/mL

CAS  NO.

83534-39-8

MDL  number

MFCD00131222

适用范围

生物试剂

l  理化信息

外 观

甘油水的缓冲溶液

l  生物学信息

生物活性/药理作用

Hydrolyzes an N₄-(acetyl-β-D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β-D-glucoaminylamine and the peptide containing an aspartic residue.

应 用

Glycopeptidase A from almonds is used for deglycosylation.It catalyzes the removal of N-ed oligosaccharide chains and converts Asn residue to Asp.

样品准备说明

Solution in 50% glycerol containing 50mM citrate-phosphate buffer, pH5.0, and BSA.

有效单位定义

One unit will hydrolyze 1.0μmole of ovalbumin glycopeptide per min at pH5.0 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov