产品名称

PNGase F from Elizabethkingia meningoseptica

别 称

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, Peptide N-glycosidase

含 量

recombinant, expressed in E. coli, set of 100units nanomolar unit; ≥10units/mg protein

CAS  NO.

83534-39-8

MDL  number

MFCD00131222

适用范围

生物试剂

l  生物学信息

生物活性/药理作用

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. PNGase Fcleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-ed core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-ed asparagine as the central residue is the minimal substrate for PNGase F.

应 用

Used to deglycosylate protein. PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

有效单位定义

One unit will catalyze the release of N-ed oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1IUB milliunit.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov