产品名称

Creatininase from microorganisms

别 称

Creatinine Amidohydrolase

含 量

100-300units/mg protein; Protein, 65-85%

CAS  NO.

9025-13-2

分子量

mol wt ~175 kDa

MDL  number

MFCD00130877

适用范围

生物试剂

l  理化信息

物理形态

Lyophilized powder containing sucrose and BSA as stabilizers

l  生物学信息

生物活性/药理作用

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

应 用

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

样品准备说明

Lyophilized powder containing sucrose and BSA as stabilizers

有效单位定义

One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH8.0 and 25°C

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov