产品名称

Creatinase from Actinobacillus sp.

一般描述

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunit and two defined domains; N and C terminal domains. C terminal fold have both the α helices and anti-parallel β sheet within two structurally similar domains. In between these two domains, there is a sulfhydryl group that acts as active site and the activity is metal independent.

别 称

Creatine Amidinohydrolase

含 量

Protein (Biuret):40-60%;

20-40 units/mg protein

CAS NO.

37340-58-2

分子量

~100 kDa

MDL number

MFCD00130875

适用范围

生物试剂

l  理化信息

外 观

lyophilized powder

物理形态

Lyophilized powder containing sugars and EDTA as stabilizers

物理性质

Isoelectric point: 4.6 ± 0.1

Michaelis constant: 1.9 x 10‾²M (Creatine)

Structure: 2 subunits per mole of enzyme

Inhibitors: Cu⁺⁺, Hg⁺⁺,   Ag⁺

Optimum pH: 8.0

Optimum temp: 40°C

pH Stability: pH 5.5 − 9.0 (25°C, 16hr)

Thermal stability: Below 50°C (pH 7.5, 30 min)

l  生物学信息

生物活性/药理作用

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

应 用

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

有效单位定义

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov