产品名称

Pyrophosphatase, Inorganic from baker's yeast (S. cerevisiae)

一般描述

Molecular Weight: 71kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)

Extinction Coefficient: E_1% =14.5 (280nm)

pI: 4.75

This ubiquitous enzyme serves to drive bolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a  lloprotease that requires Mg₂₊ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg₂₊,  both ADP and ATP can be hydrolyzed if zinc is present.

别 称

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

含 量

≥500units/mg protein (E1%/280)

CAS  NO.

9024-82-2

MDL  number

MFCD00131379

适用范围

生物试剂

l  理化信息

外 观

白色至类白色粉末

l  生物学信息

应 用

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPⁱ + H²O →  2Pⁱ.

It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase has been used:

as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.

as a component of PCR mixture

Pyrophosphatase has been used:

• as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.

• as a component of PCR mixture.

样品准备说明

Lyophilized powder containing 85% buffer salts; The lyophilized powder contains approximately 85% buffer salts (Tris, citric acid  and magnesium chloride). This product is soluble in deionized water (2mg/ml), yielding a clear and colorless solution.

有效单位定义

One unit will liberate 1.0μmole of inorganic orthophosphate per min at pH7.2 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov