Adenosine 5′-Triphosphatase from porcine cerebral cortex
商品编号：
S0794
备注：
国外库存，货期2-4周，具体详情请联系销售人员。
详细介绍
l 基本信息 |
产品名称 | Adenosine 5′-Triphosphatase from porcine cerebral cortex |
别 称 | ATP phosphohydrolase, ATPase |
含 量 | ≥0.3units/mg protein, pH7.8; Protein, 5.0-25.0% biuret |
CAS
NO. | 9000-83-3 | MDL
number | MFCD00135671 |
适用范围 | 生物试剂 |
l 理化信息 |
外 观 | 冻干粉 |
l 生物学信息 |
生物活性/药理作用 | This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and
potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate,and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells . |
应 用 | ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity . |
样品准备说明 | Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl |
有效单位定义 | One unit will liberate 1.0μmole of inorganic phosphorus from ATP per min at pH7.8 at 37°C in the presence of Na+, K+, and Mg2+. |
l 存储 |
存储温度 | -20°C |
l 注意事项及免责声明 |
本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。 |
l 参考文献 |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
