产品名称

Apyrase from potatoes

别 称

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

含 量

High Activity, ATPase ≥600units/mg protein; protein, ≥30%

CAS  NO.

9000-95-7

MDL  number

MFCD00130542

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent l ions are required for activity and best activity is observed with calcium ion at 5mM.

应 用

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).

Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The product is a high activity apyrase.

样品准备说明

Lyophilized powder containing potassium succinate buffer salts; This product is soluble in water (1mg/ml).

有效单位定义

One unit will liberate 1.0μmole of inorganic phosphate from ATP or ADP per min at pH6.5 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov