产品名称

Apyrase from potatoes

别 称

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

含 量

ATPase ≥200units/mg protein, Protein, 30-60%

CAS  NO.

9000-95-7

MDL  number

MFCD00130542

适用范围

生物试剂

l  理化信息

外 观

冻干粉

溶解性(25°C)

H²O: soluble 1.0mg/mL

l  生物学信息

生物活性/药理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent l ions are required for activity and best activity is observed with calcium ion at 5mM.

应 用

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).

Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase has been used in inhibition studies of platelet-aggregation.

样品准备说明

Partially purified, lyophilized powder containing potassium succinate buffer salts. This product is soluble in water (1mg/mL). One publication has reported preparation of 1,000units/mL stock solutions of apyrase in PBS.

有效单位定义

One unit will liberate 1.0μmole of inorganic phosphate from ATP or ADP per min at pH6.5 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov