Apyrase from potatoes
商品编号：
S0799
备注：
国外库存，货期2-4周，具体详情请联系销售人员。
详细介绍
l 基本信息 |
产品名称 | Apyrase from potatoes |
别 称 | Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase |
含 量 | ATPase ≥60units/mg protein, Protein, 30-60% |
CAS
NO. | 9000-95-7 | MDL
number | MFCD00130542 |
适用范围 | 生物试剂 |
l 理化信息 |
外 观 | 白色至深黄色或深棕色冻干粉 |
溶解性(25°C) | H2O: soluble 10mg/mL, slightly hazy |
l 生物学信息 |
生物活性/药理作用 | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis.Divalent l ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa. |
应 用 | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of
ATP involvement in intestinal lamina propria Th17 cell differentiation. |
样品准备说明 | This product is soluble in water at 10mg/mL. |
有效单位定义 | One unit will liberate 1.0μmole of inorganic phosphate from ATP or ADP per min at pH6.5 at 30°C. |
l 存储 |
存储温度 | -20°C |
l 注意事项及免责声明 |
本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。 |
l 参考文献 |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
