产品名称

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

一般描述

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.

pH optimum: ~7.9.

K^M for CO²: ~0.45mM.

Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

别 称

3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco

含 量

0.01-0.1unit/mg solid

CAS  NO.

9027-23-0

MDL  number

MFCD00130908

适用范围

生物试剂

l  理化信息

物理形态

partially purified powder

l  生物学信息

应 用

D-Ribulose 1,5-diphosphate carboxylase from spinach has been used in a study to assess catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies. D-Ribulose 1,5-diphosphate carboxylase from spinach has also been used in a study to determine RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model.

有效单位定义

One unit will convert 1.0μmole of D-RuDP and CO² to 2.0μmoles of D-3-phosphoglycerate per min at pH7.8 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov