产品名称

Carbonic Anhydrase from bovine erythrocytes

别 称

Carbonate Dehydratase, Carbonate Hydrolyase

含 量

≥2,000W-A units/mg protein

CAS  NO.

9001-03-0

MDL  number

MFCD00081467

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Carbonic anhydrase is a zinc lloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO² homeostasis, transport of bicarbonate and CO², biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore,  this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease.

应 用

Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound l ion, Zn₂₊, on aggregation behavior of the enzyme. Removal of l ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the  molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO² measurement. It has also been used for the thermodynamic analysis of conformational changes in  BCA.

有效单位定义

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov