产品名称

Carbonic Anhydrase II human

别 称

CA-II, CA2, Carbonic Anhydrase 2

含 量

recombinant, expressed in E. coli, ≥5,000units/mg protein

MDL  number

MFCD01634577

适用范围

生物试剂

l  理化信息

外 观

无色透明缓冲水溶液

l  生物学信息

生物活性/药理作用

Carbonic anhydrase is a zinc lloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO² homeostasis, transport of bicarbonate and CO², biosynthetic reactions, bone resorption, calcification, and tumorigenicity. It is required for renal acidification. Its absence can lead to osteoporosis, renal tubular acidosis, and cerebral calcification. Therefore, this enzyme is an important target for inhibitors with clinical applications in glaucoma, epilepsy and Parkinson’s disease. In addition, it is being explored as a potential target for obesity and cancer. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and facilitation of CO² elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.

应 用

Human carbonic anhydrase II has been used in a study to assess quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. Human carbonic anhydrase II has also been used in a study to investigate a new scrubber concept for catalytic CO² hydration.

样品准备说明

Supplied as a solution in 20mM Tris, pH7.5, with 150mM NaCl.

有效单位定义

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C.  (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov