产品名称

Carbonic Anhydrase II bovine

含 量

≥90%(SDS-PAGE), recombinant, expressed in E. coli, ≥5,000units/mg protein

适用范围

生物试剂

l  理化信息

外 观

无色透明缓冲水溶液

l  生物学信息

生物活性/药理作用

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid- balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications. Carbonic anhydrases are lloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA  II) is a cytosolic, single-chain, approximately 29 kDa enzyme.

应 用

Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques. Carbonic anhydrase is also used to study acid- regulation in fish and carbonic anhydrase type II deficiency syndrome . Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.

样品准备说明

Supplied as a solution in 20mM Tris, pH7.6, with 150mM NaCl.

有效单位定义

One unit will decrease th pH of a 20mM tris buffer from pH8.3 to 6.3 per minute at 0°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov