产品名称

Enolase from baker's yeast (S.cerevisiae)

别 称

2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase

含 量

≥50units/mg protein

CAS  NO.

9014-08-8

MDL  number

MFCD00131019

适用范围

生物试剂

l  理化信息

外 观

白色至类白色冻干粉末

溶解性(25°C)

15mM Tris HCl, pH7.4: soluble 1.0mg/mL, clear

l  生物学信息

生物活性/药理作用

Enolase is a lloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis. Enolase from baker’s yeast is a homodimer containing two bound Mg₂₊ ions. The molecular weight is 93.069 kDa.The peptide consists of 436 amino acids and contains a single cysteine residue. Two of the active site components include His191 and  Arg414. The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase. Apart from Mg₂₊, the enzyme can be activated by Zn₂₊, Mn₂₊, and Cd₂₊.

应 用

Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of l ions in catalysis by enolase. The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

样品准备说明

Lyophilized powder containing Tris buffer salts; This enzyme is soluble in 15mM Tris HCl, pH7.4, (1mg/ml), yielding a clear  solution.

有效单位定义

One unit will convert 1.0μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH7.4 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov