产品名称

Chondroitinase ABC from Proteus vulgaris

别 称

Chondroitin ABC Lyase

含 量

0.3-3units/mg solid (using chondroitin sulfate C as substrate); Protein,~35% biuret

CAS  NO.

9024-13-9

MDL  number

MFCD00130795

适用范围

生物试剂

l  理化信息

外 观

白色至黄褐色或浅橙色冻干粉末

l  生物学信息

生物活性/药理作用

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) ages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120kDa with 2 non-identical subunits of molecular masses 86kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37°C. 1mM Zn₂₊ acts as an inhibitor, while 0.05M acetate is an activator of the enzyme.

应 用

Chondroitinase ABC from Sigma has been used to digest O-ed chondroitin sulfate-like glycosaminoglycan (CSGAG). The  enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.

样品准备说明

Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50mM Tris, pH8.0, with 60mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.

有效单位定义

One unit will liberate 1.0μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0μmole of  2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH8.0 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov