产品名称

Chondroitinase ABC from Proteus vulgaris

别 称

Chondroitin ABC Lyase

含 量

BSA free, 50-250units/mg protein (using chondroitin sulfate C as substrate); Protein, ~10% Lowry

CAS  NO.

9024-13-9

MDL  number

MFCD00130795

适用范围

生物试剂

l  理化信息

外 观

白色至黄色冻干粉末

溶解性(25°C)

0.01% bovine serum albumin aqueous (BSA) solution: soluble

l  生物学信息

生物活性/药理作用

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl or (1-3)-α-L-iduronosyl ages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C.1 mM Zn₂₊ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

应 用

The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.

样品准备说明

Reconstitute in a 0.01% BSA aqueous solution. Subsequent dilutions can be made into a buffer containing 50mM Trizma HCl, pH8.0, with 60mM sodium acetate and 0.02% BSA. Prepare solutions just prior to use.

有效单位定义

One unit will liberate 1.0μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0μmole of  2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH8.0 at  37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov