产品名称

Heparinase I and III Blend from Flavobacterium heparinum

一般描述

Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin.

含 量

≥200units/mg protein

适用范围

生物试剂

l  生物学信息

生物活性/药理作用

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated  α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.

应 用

Heparinase I and III may be used for the study of heparin production during fermentation and specific activity of heparinise.

样品准备说明

Similar to other heparinase products, one solvent system that can be used to reconstitute this product is 20mM Trizma HCl, pH7.5, with 0.1mg/mL BSA and 4mM CaCl².

有效单位定义

One unit will form 0.1micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.One unit will form 0.1micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov