产品名称

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

一般描述

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

别 称

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

含 量

≥1,000units/mg protein (Lowry)

CAS  NO.

37288-19-0

MDL  number

MFCD00131896

适用范围

生物试剂

l  理化信息

外 观

白色至类白色冻干粉末

l  生物学信息

生物活性/药理作用

Used for the release of GPI anchored proteins from the membrane.

应 用

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

样品准备说明

The enzyme can be solubilized in 10mM Tris-HCl, pH7.4, containing 144mM NaCl and 0.05% BSA.

有效单位定义

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH7.4 at 30°C (10minute incubation).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov