产品名称

Phosphomannose Isomerase from Escherichia coli

别 称

D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI

含 量

recombinant, expressed in E. coli, ≥50units/mg protein

MDL  number

MFCD00131899

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

l  生物学信息

生物活性/药理作用

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a between glucose bolism and mannosylation.

应 用

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and l ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate  biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

样品准备说明

Supplied as a suspension in 3.2M ammonium sulfate

有效单位定义

One unit will convert 1.0μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH7.6 at 25°C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov