产品名称

Protein Disulfide Isomerase from bovine  liver

别 称

PDI

含 量

>95%(SDS-PAGE), ≥100units/mg protein, Protein, ~10% Lowry    

CAS  NO.

37318-49-3

MDL  number

MFCD00163364

适用范围

生物试剂

l  理化信息

外 观

白色至黄褐色冻干粉末

l  生物学信息

生物活性/药理作用

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.

应 用

Protein Disulfide Isomerase(PDI) is a ubiquitous, highly conserved redox chaperone enzyme from the thioredoxin superfamily. It is mainly located in the ER, where it assists in protein-folding and thiol-disulfide exchanges. It is used to study functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi.

样品准备说明

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

有效单位定义

One unit cause a change in A650 of 0.01 per min of a 1.0mg/mL solution of insulin in the presence of dithiothreitol at pH7.5 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov