产品名称

Topoisomerase I human

一般描述

Cellular topoisomerase I is present in retroviral particles and enhances viral cDNA synthesis.

含 量

≥2units/μL

分子量

mol wt 100 kDa

MDL  number

MFCD03458765

适用范围

生物试剂

l  理化信息

外 观

缓冲甘油水溶液

l  生物学信息

生物活性/药理作用

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

应 用

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

样品准备说明

Solution containing 20mM sodium phosphate, pH7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100mM imidazole (concentration will be lot dependent).

有效单位定义

One unit will relax 0.25μg of supercoiled plasmid DNA in 30 minutes at pH7.9 at 37°C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov