产品名称

DNA Gyrase from Escherichia coli

含 量

≥2unit/μL

分子量

mol wt ~374 kDa

MDL  number

MFCD00211877

适用范围

生物试剂

l  理化信息

外 观

甘油水溶液

l  生物学信息

生物活性/药理作用

Can be used to supercoil plasmids.DNA gyrase is supplied as an A²B² holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA.DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

应 用

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

有效单位定义

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37°C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov