产品名称

Pyruvate Carboxylase from bovine liver

别 称

Pyruvate:CO² ligase (ADP-forming)

含 量

5-25units/mg protein (BCA); ≥0.5mg/mL

CAS  NO.

9014-19-1

MDL  number

MFCD00132143

适用范围

生物试剂

l  理化信息

外 观

缓冲甘油水溶液

l  生物学信息

生物活性/药理作用

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

应 用

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes. The enzyme has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

有效单位定义

One unit will convert 1.0μmole of pyruvate and CO² to oxalacetate per min at pH7.8 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov