产品名称

Acetyl-CoA carboxylase 2 human

别 称

ACACB, ACC2, acetyl-CoA carboxylase beta

含 量

recombinant, expressed in Sf9 cells; ≥25units/μg protein

适用范围

生物试剂

l  理化信息

外 观

缓冲水溶液

l  生物学信息

生物活性/药理作用

Acetyl-CoA Carboxylase (ACC) regulates the bolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA  through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation. The Acetyl-CoA Carboxylase enzymes are activated by citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Because of thier roles in fatty acid bolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and bolic syndrome disorders.

应 用

Acetyl-CoA carboxylase is responsible for synthesis of Malonyl-CoA which is an inhibitor of fatty acid oxidation in skeletal muscle mitochondria. The enzyme may be used to study the effect on production of malonyl-CoA as well as fatty acid oxidation during exercise. The enzyme also may be used for ACC regulation study in anti-obesity and anti-type 2 diabetes therapeutics.

样品准备说明

Buffered aqueous solution containing Tris-HCl, pH8.0, NaCl, glycerol, and DTT. May also contain one or more of the following: EDTA, KCl, imidazole, TWEEN-20.

有效单位定义

One unit will cause the carboxylation of 1 picomole of acetyl-CoA per minute at pH7.4 at 30 deg C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov