产品名称

Melittin from honey bee venom

一般描述

Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct haemolytic agent.† The encoded protein containins 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840 Daltons and tetrameric form has molecular weight of approximately12,500 Daltons.

别 称

Melittin;Bee venom melittin

纯 度

≥85%(HPLC)

CAS NO.

20449-79-0

分子式

C¹³¹H²²⁹N³⁹O³¹

分子量

2846.46

氨基酸序列

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂

适用范围

生物试剂，适用于细胞培养等

l  理化信息

外 观

白色至淡淡的棕褐色或淡黄色冻干粉末

溶解性

水

≥5mg/mL

l  生物学信息

生物活性/药理作用

Binds calmodulin in a Ca₂₊-dependent  manner; inhibits Na₊-K₊-ATPase.Melittin acts as an  anti-coagulating protein by increasing the time of blood clotting in vitro.  Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment.

应 用

Melittin from honey bee venom has been used:

• In 3-(4, 5-dimethyl  thiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay to determine its  cytotoxicity effect on the growth of human cell lines.

• To study the anti-microbial activity of  melittin on the growth of Borrelia burgdorferi in in vitro conditions.

• As a positive control in hemolysis assay and as a cytotoxic agent against HeLa cells.

l  存储

存储温度

−20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov